Dako Autostainer Bedienungsanleitung

Dako Autostainer | User Guide Document Number 0003106 Revision E March 2007 For In Vitro Diagnostic Use UL and cUL Dako Color

10 Dako Autostainer | User Guide Dako Autostainer Features Universal System The Dako Autostainer is a universal system enabling the direct transfer

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Dako Autostainer | User Guide 101 Section 18 | Reagent Tracking Reagent Tracking allows you to monitor the total volume of each reagent that

102 Dako Autostainer | User Guide Remove Reagent from the Reagent Tracking Log Reagents that are outdated or not critical to tracking can be remove

Dako Autostainer | User Guide 103 Section 19 | Wash Buffers Wash buffers are required for all immunohistochemical staining procedures. They f

104 Dako Autostainer | User Guide Reagent Preparation One Liter of Tris-Buffered Saline Solution (S3001) 1. Add 1L (1000 mL) of deionized water t

Dako Autostainer | User Guide 105 Shelf Life and Storage TBS solutions should be stored at 2-8C to inhibit bacterial growth. TBS solutions that

106 Dako Autostainer | User Guide 6. The final solution is 0.5M Tris-HCl, 1.5M NaCl, buffered saline, pH 7.6 with 0.5% Tween 20. One Liter of Tri

Dako Autostainer | User Guide 107 Dako Phosphate Buffered Saline Dako Phosphate Buffered Saline Solution can be used for Autostainer reagent prep

108 Dako Autostainer | User Guide Phosphate Buffered Saline The following is a procedure for the formulation of 20mM Phosphate, 150mM NaCl, buffer

Dako Autostainer | User Guide 109 Shelf Life and Storage PBS solutions should be stored at 2-8C to inhibit bacterial growth. PBS solutions that

Dako Autostainer | User Guide 11 Section 2 | System Specifications The Dako Autostainer is a combination of hardware and software integrated

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Dako Autostainer | User Guide 111 Section 20 | Troubleshooting Solving Autostainer Problems Symptom Possible Cause Solutions Autostainer w

112 Dako Autostainer | User Guide Symptom Possible Cause Solutions Buffer or DI Water does not flow out during a run or a prime. (Cont.) Inlet

Dako Autostainer | User Guide 113 Symptom Possible Cause Solutions Reagent probe is not dispensing reagent. (Cont.) Clogged reagent probe. C

114 Dako Autostainer | User Guide Symptom Possible Cause Solutions No Staining Insufficient reagent in reagent vial. The Autostainer is equ

Dako Autostainer | User Guide 115 Solving Software Problems Symptom Possible Cause Solutions User is unable to access many of the functions in

116 Dako Autostainer | User Guide Solving Computer Problems Symptom Possible Cause Solutions The computer will not turn on. The computer is u

Dako Autostainer | User Guide 117 Solving Monitor Problems Symptom Possible Cause Solutions Monitor will not turn on or goes blank intermitten

118 Dako Autostainer | User Guide Symptom Possible Cause Solutions Image on the desktop is grainy and the color is incorrect. (Cont.) Color dep

Dako Autostainer | User Guide 119 Solving Printer Problems Symptom Possible Cause Solutions The printer does not respond to print command.

12 Dako Autostainer | User Guide Slide Rack is a slide carrier holding 12 microscope slides in a horizontal position. The instrument holds a maxim

120 Dako Autostainer | User Guide Symptom Possible Cause Solutions All the printer LEDs are on steadily (not blinking). The printer has a hardw

Dako Autostainer | User Guide 121 Solving Seymour Label Printer Problems Symptom Possible Cause Solutions The text of the label does not begi

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Dako Autostainer | User Guide 123 Section 21 | Warnings and Symbols Keep the cover closed during operation. The robotic arm will move unexpe

124 Dako Autostainer | User Guide Contact your local Dako representative prior to using non-Dako reagents or solutions on your Autostainer. Some s

Dako Autostainer | User Guide 125 Section 22 | General Limitations Immunohistochemistry is a multi-step diagnostic process that requires speci

Dako Autostainer | User Guide 13 Hardware Specifications Dako Autostainer Dimensions 1.02m W x 0.69m D x 0.61m H 40" W x 27" D x 24&qu

14 Dako Autostainer | User Guide Software Specifications Operating System Windows 95/98, or equivalent. Operating Logic Designed to calculate the

Dako Autostainer | User Guide 15 Section 3 | Installation Requirements For installation of the Dako Autostainer the following requirements sh

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Dako Autostainer | User Guide 17 Section 4 | Typical Operation A few “off instrument” preparations will make the staining run more time-effic

18 Dako Autostainer | User Guide 14. Select the PRINT button to print the Reagent Layout Map. 15. Load reagents using the Reagent Layout Map. 16. S

Dako Autostainer | User Guide 19 Section 5 | Programming Overview The Dako Autostainer is a computer controlled system. The PROGRAMMING GRID

2 Dako Autostainer | User Guide Dako Autostainer Information Dako Autostainer Model: _______________________ Serial Number: ______________

20 Dako Autostainer | User Guide Menu Bar Options Slides Function The Slides option may be used as an alternative to expedite programming by reducin

Dako Autostainer | User Guide 21 Edit Lists Menu The Edit Lists menu can be accessed by moving the mouse pointer to Edit lists on the menu bar an

22 Dako Autostainer | User Guide Auto Function The Auto function can be accessed by moving the mouse pointer to Auto on the menu bar and pressing th

Dako Autostainer | User Guide 23 Section 6 | Accessing the Dako Autostainer Software The SIGN IN screen is the first screen that appears afte

24 Dako Autostainer | User Guide NOTE: In the example below, the INITIALIZE button is displayed, indicating this user is set at high security level

Dako Autostainer | User Guide 25 Section 7 | Initializing the Dako Autostainer The INITIALIZE screen is used to establish and update default

26 Dako Autostainer | User Guide The buttons on the INITIALIZE screen and their respective functions are: SLIDE LABELS displays the DESIGN SLIDE L

Dako Autostainer | User Guide 27 Editing Institution Information 1. Select INITIALIZE on the MAIN MENU screen. The INITIALIZE screen is displaye

28 Dako Autostainer | User Guide 5. Type in the same password again and press ENTER. The High security level option becomes highlighted. If a diffe

Dako Autostainer | User Guide 29 Deleting User Information 1. Select INITIALIZE on the MAIN MENU screen. The INITIALIZE screen is displayed with

Dako Autostainer | User Guide 3 Contact Dako Corporate Headquarters Denmark Tel. 45 44 85 95 00 Fax 44 85 95 95 www.dako.com Distributo

30 Dako Autostainer | User Guide Deleting Doctor Information 1. Select INITIALIZE on the MAIN MENU screen. The INITIALIZE screen is displayed with

Dako Autostainer | User Guide 31 Entering Default Reagent Information Assigning Default Reagent Dispense Volumes 1. Select INITIALIZE on the MAI

32 Dako Autostainer | User Guide Cleaning Schedule This selection determines the number of slides the instrument will stain prior to prompting the

Dako Autostainer | User Guide 33 for each field is the data entered in the SLIDE INFORMATION screen and appearance of the Programming Grid. NOTE:

34 Dako Autostainer | User Guide 3. Select the desired option for the field. The list box disappears and the selected option is displayed in the fi

Dako Autostainer | User Guide 35 NOTE: The communication port for the printer is identified using the Port box on the DESIGN SLIDE LABEL screen.

36 Dako Autostainer | User Guide 2. Select the items to be included on the Program Grid. Any or all of the listed options can be marked. Only check

Dako Autostainer | User Guide 37 Delayed Start Water Rinse: For a delayed start, idle rinses may be performed with water or buffer. Select the

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Dako Autostainer | User Guide 39 Section 8 | Entering Slide Information Slides can be programmed from the PROGRAMMING GRID either through the

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40 Dako Autostainer | User Guide Deleting a Slide When using the Slides option to program a staining run, a slide can be deleted if there is at lea

Dako Autostainer | User Guide 41 The buttons on the SLIDE INFORMATION screen and their functions are: FINISH ENTRY saves the entered information

42 Dako Autostainer | User Guide To select a Tissue that was entered at the INITIALIZE screen, press the DOWN arrow key on the keyboard until the ti

Dako Autostainer | User Guide 43 6. Select the FINISH ENTRY button to save the changes. The PROGRAMMING GRID is displayed. Select the CANCEL butto

44 Dako Autostainer | User Guide Slide # 9. Select the FINISH ENTRY button to save changes. The PROGRAMMING GRID is displayed. Select the CANCEL but

Dako Autostainer | User Guide 45 Section 9 | Designing a Protocol Protocol templates are defined as a sequential series of steps required to sta

46 Dako Autostainer | User Guide Protocol Elements The Protocol Elements are categories of reagents used to build the Protocol Outline in the PROT

Dako Autostainer | User Guide 47 Substrate-Batch This step splits the staining run into two distinct operations. The first includes all steps prio

48 Dako Autostainer | User Guide 5. Additional steps can be inserted in the Protocol Outline by highlighting a step in this column and then clickin

Dako Autostainer | User Guide 49 NOTE: When assigning a new template to an existing programmed staining run, a dialog box appears if the new tem

Dako Autostainer | User Guide 5 Table of Contents Section 1 | Introduction ...

50 Dako Autostainer | User Guide Using a New Protocol Template Without Saving It 1. After a Protocol Template has been created or modified, click o

Dako Autostainer | User Guide 51 5. Press ENTER or click on the OK button. The PROTOCOL TEMPLATE DESIGN screen appears with the selected Auto Pro

52 Dako Autostainer | User Guide Selecting and Editing the Reagent Dispense Volume for a Specific Step in a Protocol 1. Select a Protocol Template

Dako Autostainer | User Guide 53 Section 10 | Programming Slides The Autostainer can process a maximum of 48 microscope slides in a single st

54 Dako Autostainer | User Guide 2. Select the desired reagent from the list using the UP and DOWN arrow keys on the keyboard, the mouse pointer, t

Dako Autostainer | User Guide 55 Assigning Positive and Negative Control Reagents Positive and negative control reagents can be automatically add

56 Dako Autostainer | User Guide 2. In a rinse column, position the mouse pointer over a tile with a (blue) rinse buffer icon and click the right m

Dako Autostainer | User Guide 57 3. Select Auto from the menu bar on the PROGRAMMING GRID using the mouse pointer. 4. Select Setup from the

58 Dako Autostainer | User Guide 5. Click on the DELETE PROGRAM button. Select the YES button to delete the Auto Program and return to the SAVE AU

Dako Autostainer | User Guide 59 4. Move the mouse pointer to the slide(s) where the items are to be pasted and press the left mouse button. The

6 Dako Autostainer | User Guide Adding Slides...

60 Dako Autostainer | User Guide Replacing a reagent with skip step (no reagent added to slide): 1. Move the mouse pointer over the reagent step

Dako Autostainer | User Guide 61 Printing Options The Autostainer software allows the user to print out various reports including information o

62 Dako Autostainer | User Guide 3. To print all reagent labels, select the PRINT ALL button. To print only selected reagents, select desired reage

Dako Autostainer | User Guide 63 Immunohistochemical Report (IHC Report) 1. From the PROGRAMMING GRID, select the Print button. The WHICH REPORT

64 Dako Autostainer | User Guide Viewing Programmed Slides After the completion of reagent programming, the first SLIDE LAYOUT MAP is displayed. T

Dako Autostainer | User Guide 65 Each cell on the screen represents a microscope slide. Cells contain the position number, case number and primar

66 Dako Autostainer | User Guide 2. Selecting multiple dispense locations: To assign multiple dispense locations on a slide, press the CTRL key whi

Dako Autostainer | User Guide 67 ALL SLIDES Tool At the upper left corner of the SLIDE LAYOUT MAP:PROGRAM SLIDES screen is an ALL SLIDES tool. T

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Dako Autostainer | User Guide 69 Section 11 | Reagent Management Reagent lists are defined using the commands on the Edit lists menu on the P

Dako Autostainer | User Guide 7 ALL SLIDES Tool ...

70 Dako Autostainer | User Guide 3. Type the full name of the reagent you want to add to the current list and press TAB or ENTER. The software allo

Dako Autostainer | User Guide 71 Adding Primary Antibodies A primary antibody can be added to the reagent list with a specific pretreatment reag

72 Dako Autostainer | User Guide 5. If the primary antibody is a step that is preformed off the Autostainer (i.e. overnight incubation or requires

Dako Autostainer | User Guide 73 13. Enter the reagent incubation time and press TAB or ENTER. NOTE: If Manual Step was selected, the incubati

74 Dako Autostainer | User Guide 3. Display the specific reagent by selecting the scroll bar in the Reagent Name box or press the UP or DOWN arrow

Dako Autostainer | User Guide 75 Printing Reagent Labels Printing reagent labels from a programmed run. 1. Program or retrieve a previously sa

76 Dako Autostainer | User Guide Compatibility Check A compatibility check has been built into the Dako Autostainer software to minimize programmi

Dako Autostainer | User Guide 77 Every reagent in the reagent categories lists (Protocol Element) can be programmed with an appropriate compatibil

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Dako Autostainer | User Guide 79 Section 12 | Loading Reagents After programming is completed the Autostainer calculates the time required fo

8 Dako Autostainer | User Guide Printing only selected reagents from the Reagent Tracking Report: ... 101 Remove Reagent fro

80 Dako Autostainer | User Guide If more than 32 reagent vials are required by the run, a SECOND RACK button becomes available at the bottom of the

Dako Autostainer | User Guide 81 Missing Reagent Error The Dako Autostainer prompts the user when an insufficient volume of reagent is measured

82 Dako Autostainer | User Guide Partial Reagent Application Reagent vials are loaded on the Autostainer and a run has been started. 1. The reagen

Dako Autostainer | User Guide 83 Section 13 | Loading Slides After programming is completed and the reagents have been loaded, you are ready

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Dako Autostainer | User Guide 85 Section 14 | Starting a Run After the specimen-containing slides and reagent vials have been loaded, it is a

86 Dako Autostainer | User Guide Starting a Run without Delay Starting a staining run immediately. 1. Select the NEXT button on the SLIDE LAYOUT M

Dako Autostainer | User Guide 87 5. Select the START RUN button. The RUN LOG screen is displayed. The Run Log is visible throughout the run and di

88 Dako Autostainer | User Guide Total buffer calculation Gray scroll bar Delaying the Start of a Run Starting a staining run at a later time. 1.

Dako Autostainer | User Guide 89 4. Select the START RUN button. The RUN LOG screen is displayed. The Run Log is visible throughout the run and di

Dako Autostainer | User Guide 9 Section 1 | Introduction The Dako Autostainer System is an automated slide processing system compatible with

90 Dako Autostainer | User Guide 2. Select the START RUN button. The RUN LOG screen is displayed. The elapsed, remaining and total times are listed

Dako Autostainer | User Guide 91 Section 15 | Completing a Staining Run During a staining run the RUN LOG screen is displayed and updated wit

92 Dako Autostainer | User Guide NOTE: If the Print IHC button is not pressed, then a Run Log Report will not be printed. To print out an IHC repo

Dako Autostainer | User Guide 93 Printing a Temporary Run Log If the Autostainer comes to an unexpected stop (i.e. power failure), a temporary c

94 Dako Autostainer | User Guide NOTE: If the Autostainer is stopped when there is reagent in the probe, it will ask you if you would like to clean

Dako Autostainer | User Guide 95 Section 16 | Programming During Staining Run This feature allows the user to program subsequent runs while t

96 Dako Autostainer | User Guide Reviewing the Current Program during a Staining Run This feature allows the user to review the PROGRAMMING GRID fo

Dako Autostainer | User Guide 97 Section 17 | System Maintenance It is recommended that the Dako Autostainer be cleaned after 150 slides have

98 Dako Autostainer | User Guide NOTE: If running protocols that require more frequent cleaning (i.e. using hematoxylin counterstain or high salt b

Dako Autostainer | User Guide 99 Slide Rack Cleaning Dako recommends additional cleaning for slide racks. 1. Soak the slide racks overnight in